What would be the preferred method to adjust the gain then? I'm unsure how to adjust the gain of the Op-amp.
The gain of the opamp is the ratio of R8/R9. If the value of R9 is reduced then the value of C4 must be increased to maintain low frequency sensitivity.
Just to check, the audio line signal goes into C4?
Yes. In my project it is driven from the output of my mic preamp.
In your circuit the output of an ipod or MP3 player will not be high enough without adding some gain.
Connect a volume control to the input of C4 if you want.
Many thanks